Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers |
|---|---|---|---|
| Gene (Homo sapiens) | OTX2 | NA | HGNC:8522 |
| Gene (H. sapiens) | LMX1A | NA | HGNC:6653 |
| Gene (H. sapiens) | FOXA2 | NA | HGNC:5022 |
| Gene (H. sapiens) | ASCL1 | NA | HGNC:738 |
| Gene (H. sapiens) | NGN2 | NA | HGNC:13805 |
| Gene (H. sapiens) | NURR1 | NA | HGNC:7981 |
| Gene (H. sapiens) | PITX3 | NA | HGNC:9006 |
| Cell line (H. sapiens) | hNP1 | EMD Millipore | EMD Millipore:SCR055; RRID:CVCL_GS51 |
| Antibody | anti-FOXA2 (mouse monoclonal) | Abcam | Abcam:ab60721 |
| Antibody | anti-MAP2 (chicken polyclonal) | Abcam | Abcam:ab5392 |
| Antibody | anti-Tyrosine Hydroxylase (rabbit polyclonal) | Abcam | Abcam:ab112 |
| Sequence-based reagent | VMAT2 (TaqMan assay) | Thermo Fisher Scientific | Thermo Fisher Scientific:Hs00996835_m1 |
| Sequence-based reagent | GAPDH (TaqMan assay) | Thermo Fisher Scientific | Thermo Fisher Scientific:Hs02758991_g1 |
| Sequence-based reagent | OTX2 (TaqMan assay) | Thermo Fisher Scientific | Thermo Fisher Scientific:Hs00222238_m1 |
| Sequence-based reagent | PITX3 (TaqMan assay) | Thermo Fisher Scientific | Thermo Fisher Scientific:Hs01013935_g1 |
| Sequence-based reagent | FOXA2 (TaqMan assay) | Thermo Fisher Scientific | Thermo Fisher Scientific:HS00232764_m1 |
| Sequence-based reagent | LMX1A (TaqMan assay) | Thermo Fisher Scientific | Thermo Fisher Scientific:Hs00892663_m1 |
| Sequence-based reagent | TH (TaqMan assay) | Thermo Fisher Scientific | Thermo Fisher Scientific:Hs0016594_m1 |
| Sequence-based reagent | MAP2 (TaqMan assay) | Thermo Fisher Scientific | Thermo Fisher Scientific:Hs00258900_m1 |
| Sequence-based reagent | NURR1 (TaqMan assay) | Thermo Fisher Scientific | Thermo Fisher Scientific:Hs00443062_g1 |
| Other | DAPI stain | Thermo Fisher Scientific |