Key resources table.
| Reagent type | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Mouse monoclonal anti-GFP | Cell Signaling | Catalog # 29565 | dil. 1:100 |
| Antibody | Rabbit polyclonal anti-V0a1 | Synaptic Systems | Catalog # 109 002 | dil. 1:100 |
| Antibody | Mouse monoclonal anti-Synapsin 1 | EMD Millipore | Catalog # MABN894 | dil. 1:1000 |
| Antibody | Mouse monoclonal anti-GDI | Synaptic Systems | Catalog # 130 011 | dil. 1:5000 |
| Chemical, compound, drug | 6-Cyano-7-nitroquinoxaline-2,3-dione disodium salt hydrate (CNQX) | Sigma-Aldrich | Catalog # C239 | 10 µM |
| Chemical, compound, drug | D(−)−2-Amino-5-phosphonopentanoic acid (AP-5) | Sigma-Aldrich | Catalog # A8054 | 50 µM |
| Chemical, compound, drug | Tetrodotoxin (TTX) | Enzo Life Sciences | Catalog # BML-NA120-0001 | 1 µM |
| Chemical, compound, drug | Picrotoxin (PTX) | Sigma-Aldrich | Catalog # P1675 | 50 µM |
| Chemical, compound, drug | Folimycin from Streptomyces sp. | Calbiochem/EMD | Catalog # 344085 | 200 nM |
| Chemical, compound, drug | Trypsin from bovine pancreas | Sigma-Aldrich | Catalog # T9935 | |
| Chemical, compound, drug | DNase | Sigma-Aldrich | Catalog # D5025-375KU | |
| Chemical, compound, drug | Matrigel | Corning | Catalog # 354234 | dil. 1:25 |
| Chemical, compound, drug | FuGENE 6 | Promega | Catalog # E2692 | |
| Chemical, compound, drug | QX-314 | EMD-Millipore | Catalog # 552233 | |
| Cell line | Highly transfectable derivative of human embryonic kidney-293 epithelial adherent cells (HEK293T) | ATCC | Catalog # CRL-1573 | |
| Organism/Strain | Sprague-Dawley rat pups (P2-P4) | |||
| Recombi-nant DNA | Plasmid: pRSV-REV (lentiviral packaging) | Addgene | Catalog # 12253 | |
| Recombi-nant DNA | Plasmid: pCMV-VSV-G (lentiviral packaging) | Addgene | Catalog # 8454 | |
| Recombi-nant DNA | Plasmid: pMDLg/pRRE (lentiviral packaging) | Addgene | Catalog # 12251 | |
| Recombi-nant DNA | Plasmid: pFUGW-vGlut1-pHGFP | Voglmaier et al. (2006) | N/A | |
| Recombi-nant DNA | Plasmid: pFU-Syp1-pHTomato |
Li and Tsien (2012)
Leitz and Kavalali (2011) |
N/A | |
| Recombi-nant DNA | Plasmid: pFUGW-Va01 | (see Materials and methods section) | ||
| Recombi-nant DNA | Plasmid: pFUGW-Va01-pHluorin | (see Materials and methods section) | ||
| Software and Algorithms | Forward-backward non-linear filter: noise reduction (NoiseReduc) function for Matlab from Nigel Reuel |
Chung and Kennedy (1991)
Reuel et al. (2012) |
http://web.mit.edu/stranogroup/index.php/resources/19-simulation-and-analysis-codes/norse-code-files/40-norse-algorithm.html | |
| Software and Algorithms | Single-vesicle-fusion-events | Present work | https://github.com/nchanaday/Single-vesicle-fusion-events | Copy archived at https://github.com/elifesciences-publications/Single-vesicle-fusion-events |