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. Author manuscript; available in PMC: 2018 Aug 21.
Published in final edited form as: Pediatr Res. 2018 Feb 21;83(5):1049–1056. doi: 10.1038/pr.2018.6

Figure 1. HMGB1 expression in rrRSV-infected 16HBE cells.

Figure 1

HMGB1 mRNA transcripts and protein expression were measured by qPCR and Western Blot analysis in human bronchial epithelial cells infected with rrRSV at MOI of 1 for 48 h. (A) HMGB1 gene expression was upregulated at 3 h post-infection, followed by reduced expression at 6, 24 and 48 h. β-actin gene was used as the housekeeping control for transcript normalization. Data are expressed as mean ± SEM. ***P<0.001 compared with non-infected cells. (B) HMGB1 protein expression was upregulated at 6 h post-infection, before returning to baseline at 24 and 48 hours post-infection. (C) Densitometric analysis. Data are expressed as mean ± SEM. *P<0.05, **P<0.01 compared with time point 0 h. All experiments were repeated at least three times.