KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| Escherichia coli NEB-alpha | BioLabs | Cat# C2987H |
| Escherichia coli XL1Blue | Agilent/Stratagene | Cat# 200229 |
| Escherichia coli ET12567/pUB307 | Tocchetti et al., 2013 | N/A |
| Streptomyces sp. ID38640 | Maffioli et al., 2017 | DSMZ: DSM 26212 |
| Streptomyces sp. ID38673 | Maffioli et al., 2017 | N/A |
| Streptomyces ΔpumE | this paper | N/A |
| Streptomyces ΔpumG | this paper | N/A |
| Streptomyces ΔpumI | this paper | N/A |
| Streptomyces ΔpumJ | this paper | N/A |
| Streptomyces ΔpumK | this paper | N/A |
| Streptomyces ΔpumM | this paper | N/A |
| Streptomyces ΔpumN | this paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| ampicillin | Fluka | Cat# 10044 |
| Apramycin sulfate | SIGMA | Cat# A2024-5G |
| chloramphenicol | SIGMA | Cat# C0378 |
| Creatine | SIGMA | Cat# 1150320 |
| Kanamycin sulfate | Fluka | Cat# 60615 |
| guanidine acetic acid | SIGMA | Cat# G11608 |
| Nalidixic acid | SIGMA | Cat# 1451000 |
| β-D-pseudouridine | Berry&Associates | Cat# PYA11080 |
| tetracycline | Fluka | Cat# 87128 |
| thiostrepton | SIGMA | Cat# T8902 |
| 3-guanidinopropanoic | SIGMA | Cat# G6868 |
| 4-guanidinobutanoic acid | SIGMA | Cat# G6503 |
| Uridine | SIGMA | Cat# U3750 |
| AminopseudoUridine | Maffioli et al., 2017 | N/A |
| Gln-APU | Maffioli et al., 2017 | N/A |
| PUA | this paper | N/A |
| Trifluoroacetic acid | SIGMA | Cat# T62200 |
| heptafluorobutiryc acid | SIGMA | Cat# 52411 |
| Acetonitrile HPLC grade | SIGMA | Cat# 39998 |
| Critical Commercial Assays | ||
| GenElute Bacterial Genomic DNA kit | SIGMA | Cat# NA2110 |
| GenElute Gel Extraction Kit | SIGMA | Cat# NA1111 |
| EuroGold Plasmid Miniprep KIT | EuroClone | Cat# EMR500200 |
| Quick Ligation Kit | NewEngland Biolabs Inc | Cat# M22005 |
| pEASY-T1 Cloning Kit | Transgen | Cat# CT101 |
| DreamTaq Green PCR Master Mix | ThermoScientific | Cat# K1082 |
| PCRBIO HiFi Polymerase | PCRBiosystems | Cat# PB 10.41-02 |
| Deposited Data | ||
| pum gene cluster | this paper | https://www.ncbi.nlm.nih.gov/nuccore/MG266907 |
| lydicamycin gene cluster | this paper | https://www.ncbi.nlm.nih.gov/nuccore/MG459168 |
| Desferroxamine gene cluster | this paper | https://www.ncbi.nlm.nih.gov/nuccore/MG459167 |
| Recombinant DNA | ||
| pWHM3-oriT | G. van Wezel, unpublished | N/A |
| pWHM3-oriT ΔXbaI | This paper | N/A |
| pSET152 | Bierman et al., 1992 | N/A |
| Software and Algorithms | ||
| antiSMASH v3.0.1 | Weber et al., 2015 | https://antismash.secondarymetabolites.org/ |
| MIBiG database | Medema et al., 2015 | https://mibig.secondarymetabolites.org/ |
| BiG-SCAPE | https://git.wageningenur.nl/medema-group/BiG-SCAPE, | |
| Other | ||
| YL 9300 HPLC | Young Lin | N/A |
| Symmetry Shield RP18, 5 μm, 4.6 × 250 mm column | Waters | Cat# WAT200670 |
| HPLC UltiMate 3000 | Dionex | N/A |
| LCQ Fleet mass spectrometer | Thermo scientific | N/A |
| Atlantis T3 C18 5 μm × 4.6 mm × 50 mm column | Waters | Cat# 186003744 |