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A) Delmarine binds to iron in phosphate buffered saline as assessed by UV-Vis spectroscopy. B) delmarine binds to iron with 3:1 ligand:metal stoichiometry as assessed by titration of 20 μM delmarine with increasing concentration of ferric ammonium citrate and absorbance at 330 nm. C) delmarine shows maximal absorbance at 330 nm at a ligand:metal ratio of 3:1 by the method of continuous variations.
