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. Author manuscript; available in PMC: 2019 May 18.
Published in final edited form as: ACS Chem Biol. 2018 Apr 17;13(5):1142–1147. doi: 10.1021/acschembio.8b00024

Figure 1.

Figure 1

Platform for discovery of fungal secondary metabolites and their biosynthetic pathways using fungal artificial chromosomes and mass spectrometry-based metabolomic scoring (FAC-MS). Fungal genomes are randomly sheared, and ~100 kb fragments with BGCs are inserted into FACs (top), which are A. nidulans/E. coli shuttle vectors. This enables facile deletion of biosynthetic genes (top, right). FACs are transformed into A. nidulans, a model fungus which serves as a heterologous host (bottom, at left). MS-based metabolomics is utilized to detect secondary metabolites associated with each FAC and to determine the effect of BGC mutants (bottom, at right).