Table 2.
Identification of differentially expressed and up-regulated proteins in TiK, the highly virulent T. indica isolate by MALDI-MS/MS and MASCOT.
Spot No.a | Accession IDb | Putative protein identity | Organism | Theo. pI/Mrc | Exper. pI/Mrd | Protein Scoree | Peptide no.f |
---|---|---|---|---|---|---|---|
1 | 587674393 | ATPase protein | Fusarium oxysporum | 5.93/83.36 | 3.25/40.46 | 91 | 12 |
2 | 154269657 | Glycosyl hydrolase family 45 | Beauveria bassiana | 5.37/44.85 | 3.80/38.67 | 80 | 6 |
3 | 924814796 | Hypothetical protein | Malassezia pachydermatis | 5.53/63.81 | 3.96/25.98 | 97 | 9 |
4 | 576987972 | Hsp70 protein | Rhizoctonia solani | 5.18/72.24 | 5.50/73.00 | 87 | 10 |
5 | 768764200 | Rga2p | Saccharomyces cerevisiae | 7.92/1144.45 | 6.00/44.24 | 98 | 8 |
6 | 757378465 | STE/STE7 protein kinase | Cryptococcus gattii | 7.64/64.80 | 6.50/44.24 | 88 | 15 |
7 | 448529472 | Fructose-bisphosphate aldolase | Candida orthopsilosis | 5.53/39.83 | 6.50/40.50 | 135 | 5 |
8 | 630344533 | Dyenin heavy chain | Gloeophyllum trabeum | 5.46/394.35 | 7.92/39.47 | 90 | 23 |
9 | 671695432 | Malate dehydrogenase | Lichtheimia ramose | 6.24/59.55 | 6.00/36.86 | 80 | 11 |
10 | 671695432 | Malate dehydrogenase | Lichtheimia ramose | 6.24/59.42 | 6.06/39.23 | 80 | 10 |
11 | 302894299 | Dyenin heavy chain | Cylindrobasidium torrendii | 5.42/393.86 | 6.58/39.3 | 20 | |
12 | 758984299 | Malate dehydrogenase | Ustilago maydis | 6.92/34.13 | 7.25/40.32 | 253 | 6 |
13 | 939400439 | β-1,3-Glucanosyltransferase | Ceraceosorus bombacis | 9.48/96.84 | 6.93/31.34 | 107 | 5 |
14 | 953439809 | hypothetical protein | Bipolaris victoriae | 10.52/13.37 | 7.28/26.00 | 81 | 9 |
15 | 808374308 | Glyceraldehyde-3-phosphate dehydrogenase | Pseudozyma hubeiensis | 9.11/42.08 | 8.45/40.43 | 131 | 7 |
16 | 443897433 | Glyceraldehyde 3-phosphate dehydrogenase | Pseudozyma Antarctica | 7.66/36.05 | 9.00/40.45 | 82 | 5 |
17 | 813217281 | Malate dehydrogenase | Saitoella complicata | 4.27/40.84 | 7.20/44.24 | 130 | 5 |
18 | 821061148 | Putative protein kinase | Phaeomoniella chlamydospora | 8.75/57.38 | 4.92/24.55 | 123 | 8 |
19 | 630968700 | Glycine decarboxylase | Anthracocystis flocculosa | 9.08/49.86 | 4.90/23.20 | 74 | 10 |
20 | 647401639 | CorA-like Mg2+ transporter protein | Rhodosporidium toruloides | 6.47/88.58 | 4.63/39.82 | 93 | 7 |
21 | 759210621 | hypothetical protein | Exophiala mesophila | 5.50/160.56 | 4.32/39.25 | 86 | 11 |
aDesignated Spot numbers 1–21 correspond to the 2-DE gel (Fig. 2); bthe accession number for identified proteins in NCBI non-redundant (NCBInr) database; ctheoretical mass (kDa) and pI of identified proteins from the database; dexperimental mass (kDa) and pI of identified proteins; eMascot score obtained from searching the NCBInr database; fNumber of statistically significant matched peptides.