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. 2018 May 18;9:1995. doi: 10.1038/s41467-018-04256-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — TaqI-induced DSBs and mutations induced in Arabidopsis plants. a Experimental scheme of induced genome rearrangements in diploid and tetraploid plants. Diploids and tetraploids harboring TaqI transgene are indicated as TaqI⁺(2n) and TaqI⁺(4n), respectively. b AtRAD51 mRNA expression following TaqI activation. Data represent the mean ± SEM (n = 3). c Frequency distribution of blue spots by GUS-reporter gene recombination in cotyledons of TAQed diploid [TaqI⁺(2n)] and tetraploid [TaqI⁺(4n)] plants. d,e The biomass growth phenotype increased in TAQed mutants. d Image shows control and TAQed mutant (#13, left; #19 right) plants grown in soil for 54 days, e dry weight of plants grown in soil for 14 weeks. Data represent the mean ± SEM (n = 20)