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. 2018 May 18;8:7860. doi: 10.1038/s41598-018-26128-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Levels of mRNA from PDR18 and from several ergosterol biosynthetic pathway genes during cultivation in absence or presence of acetic acid and comparison of ergosterol levels. (a) Growth curve of S. cerevisiae BY4741 in absence (○) or presence () of 60 mM acetic acid (at pH 4.0) based on culture OD_600nm. (b) Ergosterol biosynthetic pathway [adapted from KEGG (http://www.genome.jp/kegg/pathway.html)]; the ERG-genes under study are highlighted in red; (c) mRNA levels from the indicated genes by qRT-PCR, using ACT1 as the reference gene. (d) Ergosterol content in cell membranes from exponentially-growing cells of the parental and pdr18Δ strains in the absence or presence of 60 mM acetic acid (60 mM). Error bars represent standard deviation resultant from at least two biological replicates with three technical replicates each.

Inline graphic — Levels of mRNA from PDR18 and from several ergosterol biosynthetic pathway genes during cultivation in absence or presence of acetic acid and comparison of ergosterol levels. (a) Growth curve of S. cerevisiae BY4741 in absence (○) or presence () of 60 mM acetic acid (at pH 4.0) based on culture OD_600nm. (b) Ergosterol biosynthetic pathway [adapted from KEGG (http://www.genome.jp/kegg/pathway.html)]; the ERG-genes under study are highlighted in red; (c) mRNA levels from the indicated genes by qRT-PCR, using ACT1 as the reference gene. (d) Ergosterol content in cell membranes from exponentially-growing cells of the parental and pdr18Δ strains in the absence or presence of 60 mM acetic acid (60 mM). Error bars represent standard deviation resultant from at least two biological replicates with three technical replicates each.