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. 2018 May 18;8:7860. doi: 10.1038/s41598-018-26128-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Plasma membrane order of the parental and pdr18Δ strains growing in absence or presence of acetic acid, as reported by Laurdan Generalized Polarization (GP) values. (a) GP values from individual exponentially-growing cells of the parental and pdr18Δ strains cultivated either in the presence or absence of 60 mM acetic acid at pH 4.0. The boxplots represent the first and third quartiles and median for each cell population; (b) Examples of Laurdan Generalized Polarization images for the parental strain and pdr18Δ cells grown in absence or presence of acetic acid stress. Results arise from the analysis of at least 100 cells obtained from three independent assays. Asterisks represent statistical significant differences, based on t-student tests.