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. 2018 May 2;118(10):1337–1348. doi: 10.1038/s41416-018-0077-y

Fig. 4.

Fig. 4

CMKLR1-PTEN axis is responsible for downregulation of p-Akt (Ser473). a Immunofluoresent staining of PTEN and CMKLR1 in HCC cells. Scale bar = 100 µm. b PTEN–CMKLR1 interaction in chemerin-overexpressing and control cells is examined by immunoprecipitation and western blot. c Left, 7404 cell lysates of either control or chemerin-treated cells are immunoprecipitated with control IgG or anti-PTEN antibody and then subjected to western blot analysis. Right, PTEN activity in chemerin-treated cells. ***p < 0.001 for 1 h vs. 0 h, 2 h vs. 0 h, Unpaired t test. Precipitated PTEN is shown below. d Expression of CMKLR1, PTEN, p-Akt (Ser473), Akt and β-actin in control cells and CMKLR1 knockdown cells. e Ubiquitination of PTEN in control and CMKLR1 knockdown cells. f PTEN activity in control and CMKLR1 knockdown cells. ***p < 0.001 for i1/i2 vs. icon, Unpaired t test. Precipitated PTEN is shown below