Fig. 3.

Effect of methyl donor depletion on DAPK1 gene expression and protein levels. Gene expression of a DAPK1 and b PUMA in UD-SCC2 cells measured by qPCR relative to β2-microglobulin expression after 168 h depletion and 72 h repletion. c Protein expression of total DAPK1 protein and phosphorylated DAPK1 (160 kDa) along with β-actin loading control (45 kDa) in UD-SCC2 cells. In a–c, gene and protein expression was measured in UD-SCC2 cells following methyl donor depletion for 168 h with medium containing 5% methyl donors (deplete) followed by a further 72 h culture in medium containing 100% methyl donors (replete). Control cells were continuously cultured in 100% methyl donor containing medium alongside deplete and replete cells