Table 3.
Changes in growth index–Gi (calculated according to the formula: Gi = [(Dw1 − Dw0)/Dw0] where Dw1 is the dry weight of microshoots at the end of experiment and Dw0 is the dry weight of the inoculum), of S. chinensis cv. Sadova biomass from all tested in vitro systems (agar, agitated and Plantform bioreactor-grown cultures). Values represent the mean ± SD of four samples
Type of S. chinensis cv. Sadova microshoot cultures | MS medium variant* | Culture growth period | |||||
---|---|---|---|---|---|---|---|
10 days | 20 days | 30 days | 40 days | 50 days | 60 days | ||
Agar | A | 2.7 ± 0.1cdef | 2.9 ± 0.2cdef | 3.5 ± 0.1abef | 3.5 ± 0.4abef | 3.8 ± 0.3abcdf | 3.9 ± 0.6abcde |
B | 2.5 ± 0.1bcdef | 3.8 ± 0.3acdef | 4.4 ± 0.2abdef | 4.0 ± 0.6abcef | 5.2 ± 0.5abcdf | 5.7 ± 0.3abcde | |
C | 2.5 ± 0.3bcdef | 4.1 ± 0.2acdef | 3.7 ± 0.5abef | 3.7 ± 0.2abef | 7.2 ± 0.2abcdf | 5.7 ± 0.3abcde | |
D | 2.5 ± 0.1bcdef | 3.5 ± 0.5adef | 3.5 ± 0.2adef | 4.1 ± 0.5abcef | 4.9 ± 0.1abcdf | 6.3 ± 0.8abcde | |
E | 2.3 ± 0.2bcdef | 2.8 ± 0.2acdef | 3.1 ± 0.3abdef | 5.0 ± 0.6abced | 6.1 ± 0.1abcdf | 5.8 ± 0.3abcde | |
F | 2.5 ± 0.1bcdef | 3.2 ± 0.6acdef | 3.6 ± 0.3abdef | 5.6 ± 0.8abcef | 5.3 ± 0.2abcdf | 6.8 ± 0.7abcde | |
G | 2.8 ± 0.1bcdef | 3.4 ± 0.5acdef | 4.8 ± 0.6abdef | 6.8 ± 0.2abcef | 7.1 ± 0.1abcdf | 8.7 ± 0.6abcde | |
Agitated | F | 1.9 ± 0.1bcdef | 2.3 ± 0.2acdf | 2.9 ± 0.4abdef | 3.4 ± 0.5abcef | 2.3 ± 0.1acdf | 7.5 ± 0.4abcde |
G | 1.4 ± 0.1bcdef | 2.5 ± 0.2acef | 2.2 ± 0.6abdef | 2.6 ± 0.09abcef | 4.4 ± 0.2abcdf | 4.1 ± 0.2abcde | |
Plantform | G | nt | nt | 4.6 ± 0.9 4.7 ± 0.1** |
nt | nt | nt |
nt not tested
*See ‘Materials and methods’; **Gi of microshoots after elicitor (YE) treatment
ap < 0.05 vs. 10-day growth period
bp < 0.05 vs. 20-day growth period
cp < 0.05 vs. 30-day growth period
dp < 0.05 vs. 40-day growth period
ep < 0.05 vs. 50-day growth period
fp < 0.05 vs. 60-day growth period