Skip to main content
. 2018 Apr 23;102(12):5105–5120. doi: 10.1007/s00253-018-8981-x

Table 3.

Changes in growth index–Gi (calculated according to the formula: Gi = [(Dw1 − Dw0)/Dw0] where Dw1 is the dry weight of microshoots at the end of experiment and Dw0 is the dry weight of the inoculum), of S. chinensis cv. Sadova biomass from all tested in vitro systems (agar, agitated and Plantform bioreactor-grown cultures). Values represent the mean ± SD of four samples

Type of S. chinensis cv. Sadova microshoot cultures MS medium variant* Culture growth period
10 days 20 days 30 days 40 days 50 days 60 days
Agar A 2.7 ± 0.1cdef 2.9 ± 0.2cdef 3.5 ± 0.1abef 3.5 ± 0.4abef 3.8 ± 0.3abcdf 3.9 ± 0.6abcde
B 2.5 ± 0.1bcdef 3.8 ± 0.3acdef 4.4 ± 0.2abdef 4.0 ± 0.6abcef 5.2 ± 0.5abcdf 5.7 ± 0.3abcde
C 2.5 ± 0.3bcdef 4.1 ± 0.2acdef 3.7 ± 0.5abef 3.7 ± 0.2abef 7.2 ± 0.2abcdf 5.7 ± 0.3abcde
D 2.5 ± 0.1bcdef 3.5 ± 0.5adef 3.5 ± 0.2adef 4.1 ± 0.5abcef 4.9 ± 0.1abcdf 6.3 ± 0.8abcde
E 2.3 ± 0.2bcdef 2.8 ± 0.2acdef 3.1 ± 0.3abdef 5.0 ± 0.6abced 6.1 ± 0.1abcdf 5.8 ± 0.3abcde
F 2.5 ± 0.1bcdef 3.2 ± 0.6acdef 3.6 ± 0.3abdef 5.6 ± 0.8abcef 5.3 ± 0.2abcdf 6.8 ± 0.7abcde
G 2.8 ± 0.1bcdef 3.4 ± 0.5acdef 4.8 ± 0.6abdef 6.8 ± 0.2abcef 7.1 ± 0.1abcdf 8.7 ± 0.6abcde
Agitated F 1.9 ± 0.1bcdef 2.3 ± 0.2acdf 2.9 ± 0.4abdef 3.4 ± 0.5abcef 2.3 ± 0.1acdf 7.5 ± 0.4abcde
G 1.4 ± 0.1bcdef 2.5 ± 0.2acef 2.2 ± 0.6abdef 2.6 ± 0.09abcef 4.4 ± 0.2abcdf 4.1 ± 0.2abcde
Plantform G nt nt 4.6 ± 0.9
4.7 ± 0.1**
nt nt nt

nt not tested

*See ‘Materials and methods’; **Gi of microshoots after elicitor (YE) treatment

ap < 0.05 vs. 10-day growth period

bp < 0.05 vs. 20-day growth period

cp < 0.05 vs. 30-day growth period

dp < 0.05 vs. 40-day growth period

ep < 0.05 vs. 50-day growth period

fp < 0.05 vs. 60-day growth period