Fig. 2.

ALA increases the HSP70 expression under HS conditions. Caco-2 cells grown on inserts and pretreated with ALA (24h) were exposed to HS (42 °C) for 6 h (qRT-PCR) or 24 h (WB) to evaluate the expression of HSP70 in mRNA (a) and protein levels (b). Results are expressed as mRNA expression or protein expression (normalized with β-actin) relative to unstimulated cells as mean ± SEM of three independent experiments. Different lower case letters denote significant differences among groups