Fig. 3.

ALA prevents the HS-induced expression and nuclear translocation of Nrf2. Caco-2 cells grown on inserts (qRT-PCR) or 6-well plates (WB) and pretreated with ALA (24 h) were exposed to HS (42 °C) for 6 h. Results are expressed as mRNA expression (normalized with β-actin) (a) and nuclear abundance (normalized with Lamin A) (b) relative to unstimulated cells as mean ± SEM of three independent experiments. Different lower case letters denote significant differences among groups