Fig. 4.

ALA prevents the HS-induced disruption of epithelial integrity and accelerates the JC reassembly. Caco-2 cells grown on inserts were pretreated with ALA (24 h) prior to HS exposure (42 °C) and TEER (a) and LY transport (b) across the Caco-2 monolayer was measured after 24 h exposure to HS. For the calcium switch assay, Caco-2 cells were pretreated with ALA (24 h) before calcium deprivation and TEER was measured during recovery (0, 2, 4, 6, 8 h) in medium supplemented with ALA (c). Data are expressed as a percentage of initial value (TEER, calcium switch assay) or in the amount LY transported [ng/(cm² × h)] detected in the basolateral compartment of transwell inserts as mean ± SEM of three independent experiments. Different lower case letters denote significant differences among groups