Fig. 1.

Example of typical data acquired for a system suitability sample. Here, a seven component system suitability sample has been applied in a HILIC positive ion assay and includes an early elution metabolite (decanoic acid) and later elution metabolites. Leucine and isoleucine are included to assess chromatographic resolving power for isomers. The base peak chromatograms are shown for each metabolite to assess peak symmetry with retention time and m/z calculated to assess chromatographic stability and mass accuracy