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. 2018 Apr-Jun;10(2):62–68.

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Copyright© 2018 Avicenna Research Institute

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5A. — Recognition of apoptosis in MOLT-4 cells co-cultured with MSC treated with CoCl₂. Figure 5A depicts a representable sample of assessment of apoptosis using Annexing-V staining and PI. (A) Change of phosphatidylserine externalization induced by CoCl₂ in MOLT-4 cells cocultured with MSC (flow cytometry analysis, Annexin V-FITC/PI staining). MOLT-4 cells were incubated with 100 μM CoCl₂ for 24 hr. The data presents the three independent experiments. Annexin V positive/PI negative cells (Annexin V+/PI−) showed in the bottom right quadrant of each dot plot signifies cells corresponding to early apoptosis, whereas the Annexin V positive/PI positive (Annexin V+/PI+) cells showed in the upper right quadrant signifies cells corresponding to late apoptotic/necrotic cells.