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. 2018 May 18;19:367. doi: 10.1186/s12864-018-4746-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The confident BRM target genes in S2 cells. Control cells and cells stably transfected with expression vectors for recBRM-V5 and recBRM-K804R-V5 were treated with dsRNA to knock down BRM. Control cells were treated in parallel with dsRNA complementary to GFP. The expression of the recombinant proteins was induced with CuSO₄, as indicated. a Western blot analysis of BRM levels using an antibody against the endogenous BRM. Tubulin was used as a loading control. b Box plot showing the average expression levels of the BRM target genes compared with the average expression level of all the genes in S2 cells. c-d Metagene analyses of RNAPII and nucleosome occupancy in the BRM target genes (red, n = 541) and flanking sequences (X-axis) compared with the average distributions in all the S2 genes (blue, n = 13,294 genes)