Table 1.
PCR conditions for inflammatory cytokines
| Gene | Primer sequences | Annealing temperature (°C) |
|---|---|---|
| Interleukin-1-β (IL-1β) | F: 5′-CCTCCTTGCCTCTGATGG-3′ R: 5′-AGTGCTGCCTAATGTCCC-3′ |
60 |
| Tumor necrosis factor (TNF-α) | F: 5′-CCCCAGTCTGTATCCTTCT-3′ R: 5′-ACTGTCCCAGCATCTTGT-3′ |
59 |
| Interleukin-6 (IL-6) | F: 5′-GGCAATTCTGATTGTATG-3′ R: 5′-CTCTGGCTTTGTCTTTCT-3′ |
56 |
| Interleukin-8 (IL-8) | F: 5′-TGCCGTGACCTCAAGATGTGCC-3′ R: 5′-CATCCACAAGCGTGCTGTAGGTG-3′ |
60 |
| Interleukin-10 (IL-10) | F: 5′-CCAAGCCTTATCGGAAATGA-3′ R: 5′-TTCTCACCCAGGGAATTCAA-3′ |
60 |
| Glyceraldehye-3-phosphate dehydrogenase (GAPDH) | F: 5′-ATTCAACGGCACAGTCAA-3′ R: 5′-CTCGCTCCTGGAAGATGG-3′ |
56 |