Fig. 3.

FoxM1 activates ADAM-17 and promotes cell proliferation in vitro and tumor growth in vivo. a RBE cells transfected with FoxM1 and control vector after 48 h; FoxM1 and ADAM17 protein expression (upper) were detected by Western blot analysis, and cell viability was analyzed by CCK8 assay (lower). b QBC939 cells transfected with FoxM1-shRNA and scrambled control after 48 h; FoxM1 and ADAM-17 protein expression (upper) were detected by Western blot analysis, cell viability was analyzed by CCK8 assay (middle), cell cycle distribution was measured by flow cytometric analysis (lower). All the above experiments were repeated three times. c QBC939 cells (1 × 10⁷ per mouse) transfected with FoxM1-shRNA or control shRNA were subcutaneously injected into nude mice (n = 5). Resected tumor pictures (left), graphical representation of tumor volumes (right), and staining of ADAM17 in the two groups. OE, overexpression. *p < 0.05, **p < 0.01 as compared to control