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. 2018 Apr 30;115(20):E4623–E4632. doi: 10.1073/pnas.1714986115

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Fig. 4. — Gating of the C.Orai1 (FY-RH) channel is regulated by C.STIM1 binding. (A) The time course of I_CRAC development in cells expressing C.STIM1 with either C.Orai1 (blue) or C.Orai1 (FY-RH) (red) after whole-cell break-in. The Ca²⁺ permeability of the C.Orai1 (FY-RH) channel is regulated by C.STIM1 binding. (B) I–V relationships in the cells expressing C.STIM1 and either C.Orai1 (blue) or C.Orai1 (FY-RH) (red) shown in A at peak store-operated currents in 10 mM Ca²⁺ Ringer’s solution. (C) Cellular localization of Cherry-C.STIM1 and GFP-C.Orai1 (FY-RH) before store depletion. C.Orai1 (FY-RH) formed prepuncta with C.STIM1. All images of the cell footprint were taken with epifluorescence and TIRF microscopes. (Scale bar, 10 μm.) (D) FLAG-tagged C.CAD coimmunoprecipitated with both GFP-tagged C.Orai1 and C.Orai1 (FY-RH) showing that C.Orai1 (FY-RH) can be regulated by C.STIM1 or C.CAD binding.