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. 2018 Apr 30;115(20):5265–5270. doi: 10.1073/pnas.1715590115

Fig. 3.

Fig. 3.

Islet antigen reactivity of activated islet Tregs. (A) Hybridomas expressing NFATGFP reporter and TCRs derived from activated islet Tregs were stimulated for 20 to 24 h with splenic DCs or splenic DCs with islet lysates. GFP expression was determined using flow cytometry, and contour plots for all of the hybridomas analyzed are shown. (B) As described in A, except the hybridomas were stimulated with islet DCs. (C) As described in A, except the hybridomas were stimulated with splenic DCs with insulin B:9–23 peptide, plate-bound insulin tetramers p8E and p8G, human B25D-insulin, or proinsulin B:24–36 peptide. Filled histograms are the HEL-specific hybridoma; open histograms are the TCR hybridoma listed at the top of the column. In A–C, representative flow plots for at least two independent experiments are shown.