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. 2018 Apr 30;115(20):5175–5180. doi: 10.1073/pnas.1716951115

Fig. 4.

Fig. 4.

TEM observation of the integument of dsRNA-treated BPHs. BPHs injected with dsNlugCPAP3-C5b were treated at second-instar nymphs (0–12 h). BPHs injected with dsRNAs targeting the other 16 genes (NlugCpr6, NlugCpr56, NlugCpr61, NlugCpr62, NlugCpr64, NlugCpr69, NlugCpr73, NlugCpr83, NlugCPLCP2, NlugCPAP1-E, -H, -K, NlugCPAP3-D1, NlugCpr47, NlugCPAP1-N, and NlugCPAPn-7) were treated at fifth-instar nymphs (0–12 h). All of the treated BPHs were raised for the following experiments. BPHs treated with dsNlugCpr47, dsNlugCPAP1-N, and dsNlugCPAPn-7 were collected for TEM at the end of fifth-instar nymphal stage (72–78 h) when the insects were still alive; BPHs treated with dsRNA for the other genes were collected for TEM at early stage of adult females (24–30 h) while the insects were still alive. BPHs treated with dsGFP were collected at the same time as negative control animals. Images show parts of an abdominal tergum taken from the fourth to the eighth segment.