Fig. 2.

Remodeling of the glycosylation phenotype upon metabolic supplementation with GlcNAc. (A) Impact of GlcNAc supplementation on GnT-V activity was determined using a pool of lysates from treated vs. nontreated peripheral blood T cells, in three biological replicates of patients with active UC, from two independent technical experiments. Student’s t test: **P ≤ 0.01. (B) Flow cytometry evaluation of glycophenotype of T cells upon GlcNAc supplementation. (B1) Scatter plot: fold change of MFI due to staining with each lectin on T cells, in two biological replicates with different stages of disease severity (Mayo endoscopic scores 1 and 2), from two independent experiments. Results are normalized to the untreated condition, which was taken as 1. Student’s t test: *P ≤ 0.05. NS, not significant.