Expression profile of P. andersonii symbiosis genes that are lost in Trema species. Expression of symbiosis genes in P. andersonii stem, leaf, female and male flowers, lateral root primordia, roots, and three nodule stages (S1–S3), and in F1 hybrid roots and nodules (line H9). Expression is given in DESeq2-normalized read counts; error bars represent SE of three biological replicates for lateral root primordia, root, and nodule samples. Dots represent individual expression levels. PanCRK11, CYSTEINE-RICH RECEPTOR KINASE 11; PanDEF1, DEFENSIN 1; PanLEK1, LECTIN RECEPTOR KINASE 1; PanNFP2, NOD FACTOR PERCEPTION 2; PanNIN, NODULE INCEPTION; PanRPG, RHIZOBIUM DIRECTED POLAR GROWTH.