Fig. 2.

HFD treatment increases expression of the DHET-producing enzyme sEH in colon tissues. (A) Biochemistry for biosynthesis of fatty acid epoxides and diols. (B) qRT-PCR analysis of gene expression in colon tissues (n = 4 or 5 mice per group for Pla2g4a, Cyp3a11, Cyp2c29, Cyp2c38, Cyp2c37, Cyp2c39, Cyp2j5, Cyp2j6, Cyp2j8, and Cyp2j9; n = 7 mice per group for Ephx2). (C) Immunohistochemical staining showing increased sEH in colon tissues from HFD mice (magnification 600×; n = 5 for LFD; n = 8 for HFD). The results are mean ± SEM. (Scale bars: 50 μm.)