Table 1.

Numbers of injected and surviving A. millepora zygotes

	Night 1			Night 2
Target gene(s)*	FGF1a	GFP	RFP	FGF1a	None (Cas9-only)
No. of individuals injected successfully†	146	123	147	246	227
No. of individuals surviving until 12 h postfertilization‡	74	88	79	116	176
Percent of individuals surviving until 12 h postfertilization, %‡	51	72	54	47	78

^*For each gene, a mixture of sgRNA/Cas9 complexes was injected (SI Appendix, SI Materials and Methods); there were two such complexes for FGF1a, three for GFP, and two for RFP.

^†As judged by the phenol-red injection marker (SI Appendix, SI Materials and Methods).

^‡As judged by the numbers of larvae that were seemingly intact at the time of observation.