Fig. 1.

Bcl2l12 mRNA expression in WT and Irf3^−/− cells. (A) Whole-cell extracts were prepared from WT and Irf3^−/− MEFs and subjected to immunoblot analysis with anti-IRF3 and β-actin antibodies. (B) Whole-cell extracts were prepared from WT and Irf3^−/− GM-CSF–cultured BM-DCs and macrophage-CSF–cultured BMMs and subjected to immunoblot analysis. (C) MEFs were prepared from WT, Irf3^−/−Bcl2l12^−/−, or Irf3^−/− mice. Total RNA was extracted, and Bcl2l12 mRNA expression was determined by qRT-PCR analysis. ND, not detected. *P < 0.05. Results shown are mean ± SD of three independent experiments.