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. 2018 Apr 30;115(20):5253–5258. doi: 10.1073/pnas.1803936115

Fig. 2.

Fig. 2.

Ifnb mRNA induction in WT and Irf3−/− cells in response to PRR ligands or virus infection. (A–C) MEFs (A), BM-DCs (B), and BMMs (C) obtained from WT and littermate Irf3−/− mice were stimulated with poly (I:C) (10 μg/mL), B-DNA (10 μg/mL), LPS (200 ng/mL), or CpG-B ODN (1 μM) for the indicated times. Total RNA was extracted, and Ifnb mRNA expression was determined by qRT-PCR analysis. *P < 0.05. Results shown are mean ± SD from three independent experiments. (D–F) WT and littermate Irf3−/− MEFs (D), BM-DCs (E), and BMMs (F) were infected with VSV, HSV-1, EMCV, or L. monocytogenes (L. mono) at a multiplicity of infection of 1 for the indicated times. Total RNA was extracted, and Ifnb mRNA expression was determined by qRT-PCR analysis. *P < 0.05. Results are mean ± SD of three independent experiments.