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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: J Hepatol. 2018 Mar 9;68(6):1228–1238. doi: 10.1016/j.jhep.2018.01.026

Fig. 3. Identification of MCL1 species in CCA cells.

Fig. 3

(A) Isolated mitochondria from KMCH cells were digested with 50 μg/mL trypsin for 20 min and subjected to SDS-PAGE. HSP60 was used as a loading control, and TOM70 and TIMM50 were used as markers for the mitochondrial outer and inner membrane, respectively. (B) KMCH cells were treated with cycloheximide (CHX, 100 μg/mL) for 2 and 4 h. Expression of MCL1 species was examined by western blotting.