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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Immunol Methods. 2018 Apr 21;458:44–52. doi: 10.1016/j.jim.2018.04.004

Figure 1. Validation of GMM-CD1b tetramer using GMM-specific T cell lines.

Figure 1

(A) GMM-specific T cell line was tested for antigen-specificity using IFN-γ ELISPOT. T cells were incubated with K562 cells that were stably transfected with CD1b (K562 CD1b) or mock transfected with an empty vector (K562 EV) in the presence of 1 g/mL GMM followed by quantification of the number of IFN-γ spots present. (B) GMM-specific T cell line was stained with two batches of GMM-CD1b tetramer generated three months apart to assess batch-to-batch variation of the lipid-loaded tetramer. (C) Summary of GMM-CD1b tetramer staining from eight batches of tetramer prepared over 18 months. Data are depicted as a proportion of total CD3+ cells. Results in panels A and B are representative of at least eight independent experiments.