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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Immunol Methods. 2018 Apr 21;458:44–52. doi: 10.1016/j.jim.2018.04.004

Figure 2. Assessing the repeatability, intermediate precision, and reproducibility of the CD1 tetramer assay.

Figure 2

The GMM-specific T-cell line was serially diluted in PBMC from a healthy donor and stained with GMM-CD1b tetramer. (A) Summary data from three independent experiments performed by a single operator in which the mean and standard error of the mean are displayed for each dilution tested. (B) Coefficient of variation (CV) across the three experiments, where the CV value of 30% is indicated (dashed line). To assess intermediate precision, three different operators performed the CD1 tetramer assay on three different flow cytometers using the same batch of T-cell lines and GMM-CD1b tetramer. Shown are the (C) mean and standard error of the mean at each dilution as well as the (D) CV of the experiments acquired between the three operators. Data for intermediate precision are representative of one experiment.