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. Author manuscript; available in PMC: 2018 May 19.
Published in final edited form as: Anal Chem. 2018 Jan 8;90(3):1870–1880. doi: 10.1021/acs.analchem.7b03949

Figure 3.

Figure 3

Selective-trapping and -delivery strategy. (a) Rationale for the selective-trapping and -delivery strategy that enhances selectivity, sensitivity, robustness, and throughput. (b) Development of the optimal trapping (high pH) and delivery (low pH) conditions for the 10 proteins in the biomatrices. (c) Recoveries of the 3 target SP (colored lines) vs those of the 42 representative coretrieved liver matrix peptides (gray lines, a detailed list of these peptides are in Table S2) under different loading and delivery conditions. (d) Example showing the recoveries of a target SP (AB095) vs those of the matrix peptides after selective trapping and delivery (n = 3). (e) Percentage distributions of the peptide masses that entered the μLC-MS system and those of the SPs removed by selective trapping and delivery.