Fig. 4.

AATYK knockdown does not affect the survival but maintains proliferation of OPCs. A Ki67 immunostaining showed there were more Ki67⁺ and GFP⁺ co-labeled cells in the sh-AATYK-treated OPCs than in the control. B Percentages of Ki67⁺ and GFP⁺ co-labelled OLs in GFP⁺ cells among RNAi-AATYK cells were significantly higher than in the control. C–D Compared to control cells, the expression of MBP protein was significantly decreased in shRNA-AATYK-treated cells and there was no difference in caspase3 between the two groups. Scale bar, 100 μm.