Figure 2.

Multiple displacement amplification process and chimera types. (A) Primer Phi29 DNA polymerase annealing to the DNA and extension by Phi29 DNA polymerase. Phi29 DNA polymerase can displace downstream 5′-termini DNA strand to extend the growing 3′-termini strand by a simple branch migration reaction, and 3′-termini can be displaced as well in a similar way. (B) Four types of chimeric rearrangements. I: the second segment is inverted from its original orientation and directly joined after the first segment. II: the second segment is inverted from its original orientation and directly joined before the first segment. III: two directed segments are directly joined. IV: two directed segments are reversely joined