Fig. 3.

Whole-cell currents evoked by JCT in human colonic Caco-2 cells. a, c Representative time courses of the JCT-evoked whole-cell currents recorded at +100 and −100 mV under ramp clamp. Gray bar and solid bar show application of 400 μg/mL of JCT and 20 μM of CFTR inhibitor-172 (CFTR-inh.), respectively. b, d Corresponding I–V relationships at time points a, b and c. e Averages of JCT-induced whole-cell current in control and CFTR inh. (n = 5–6). The inset shows JCT-induced whole-cell current when the extracellular Cl⁻ concentration is reduced from control to 55 mM CsCl (55 Cs-Cl) bath solution. Data points show the mean ± SEM. ***P < 0.001 compared to control at +100 mV