Table 1.
Clones selection after fusion | Clones selection after the first subcloning | ||
---|---|---|---|
Clones | OD at 450 nm | Clones | OD at 450 nm |
Neg. ctrl | 0.051 | Neg. ctrl | 0.050 |
Pos. ctrl | 3.46 | Pos. ctrl | 2.360 |
1-E4 | 3.042 | 1-1E4 | 2.26 |
4-B10 | 3.051 | 4-1B10 | 1.796 |
5-E2 | 3.867 | 5-1E2 | 2.233 |
5-E12 | 3.654 | 5-1E12 | 2.133 |
6-G9 | 3.676 | 6-1G9 | 2.4 |
8-D10 | 3.642 | 8-1D10 | 0.057 |
8-F8 | 3.846 | 8-1F8 | 0.055 |
8-H11 | 3.533 | 8-1H11 | 0.07 |
Neg. ctrl: negative control, serum from nonimmunized mouse. Pos. ctrl: positive control, serum from immunized mouse.