Wild-type human p23 coding sequence from nucleotide 301 to 360 is shown, with translation start site as +1. Amino acid (aa) 106 to 112 represents the region where point mutants W106A, W109A, D108A, D111A, and D112A were generated. Sense mutagenic primers are shown with the mutated codons bolded and the nucleotide numbers. Lines represents the length of the primers. The antisense mutagenic primers are the reverse complement of the sense primers.