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. Author manuscript; available in PMC: 2019 Jun 1.

Published in final edited form as: Biochem Pharmacol. 2018 Mar 17;152:34–44. doi: 10.1016/j.bcp.2018.03.015

Fig. 2 — Down-regulation of the p23 protein decreased the AHR protein and message levels in HeLa cells. (A) Western and qPCR results showing that AHR protein and message levels were reduced in p23 knockdown (p23KD) stable HeLa cells when normalized to the wild-type (WT) HeLa cells (arbitrarily set as 1). 1 μg of RNA from HeLa or p23KD cells was used to follow the qPCR protocol described under 2.6. The percentages represent the % content remaining. (B) Western results showing the amount of AHR after cycloheximide (40 μg/ml) treatment was plotted with zero time point arbitrarily set as one. All plots (A and B) are quantified data (means ± SD, n = 3 for Western data whereas n = 5 for qPCR data). The images are representative of replicate data. Each Western lane contained 40 μg of protein.

Fig. 2 — Down-regulation of the p23 protein decreased the AHR protein and message levels in HeLa cells. (A) Western and qPCR results showing that AHR protein and message levels were reduced in p23 knockdown (p23KD) stable HeLa cells when normalized to the wild-type (WT) HeLa cells (arbitrarily set as 1). 1 μg of RNA from HeLa or p23KD cells was used to follow the qPCR protocol described under 2.6. The percentages represent the % content remaining. (B) Western results showing the amount of AHR after cycloheximide (40 μg/ml) treatment was plotted with zero time point arbitrarily set as one. All plots (A and B) are quantified data (means ± SD, n = 3 for Western data whereas n = 5 for qPCR data). The images are representative of replicate data. Each Western lane contained 40 μg of protein.