Skip to main content
. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Biochem Pharmacol. 2018 Mar 17;152:34–44. doi: 10.1016/j.bcp.2018.03.015

Fig. 8.

Fig. 8

Down-regulation of p23 increased ubiquitination of AHR in HeLa cells. Western data showing that ubiquitinated AHR (AHR-Ub) could be observed when wild-type HeLa (WT) cells were treated with 3-MC for 2 h in the presence of 10 μM MG132 (lane 2) and also when the p23KD HeLa cells were treated with 10 μM MG132 without ligand treatment (lanes 3 and 7). Lanes 1–3 and 4–7 are images from two separate Western experiments. Anti-ubiquitin IgG was used to detect the signals. Western of lanes 1–3 was repeated once with similar results (n = 2). Experiment to detect the ubiquitinated AHR in HeLa (WT) and p23 knockdown stable HeLa cells (p23KD) ± 10 μM MG132 (lanes 4–7) was repeated twice and was plotted below the representative images (n = 3, means ± SD).