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Method to screen for CaM binding domains in AT1R. (A & B) Design of FRET biosensors to screen for CaM interaction (see text for explanation), depicted in unbound (A) and CaM-bound (B) conditions. EYFPc, citrine enhanced yellow fluorescence protein; ECFP, enhanced cyan fluorescence protein; N and C, N-terminal and C-terminal ends, respectively, of fluorophores. (C) Representative Coomassie gel of the biosensors generated. (D) Coomassie gel of three separate CaM preps.
