. Author manuscript; available in PMC: 2019 Jun 1.

Published in final edited form as: Biochem Pharmacol. 2018 Mar 30;152:187–200. doi: 10.1016/j.bcp.2018.03.027

Table 3.

Gblock gene fragments. Substitutions from wild-type sequenced are in bold-face fonts and italicized. Identified CaM binding domain sequences are underlined.

AT₁R Fragment	gBlock sequence	Restriction enzymes to clone to full-length AT₁R
125–141 mut	CTACTCACGTGTCTCAGCATTGATCAAGCCCTGGCTATTGTTCACCCAATG AAGTCCCAACTTCGACAAACAATGCTTGTAGCCAAAGTCACCTGCATCAT CATTTGGCTGCTGGCAGGCTTGGCCAGTTTGCCAGCTATAATCCATCGAA ATGTATTTTTCATTGAGAACACCAATATTACAGTTTGTGCTTTCCATTATG AGTCCCAAAATTCAACCCTCCCGATAGGGCTGGGCCTGACCAAAAATATA CTGGGTTTCCTGTTTCCTTTTCTGATCATTCTTACAAGTTATACTCTTATTG CGAAGGCCCTAAAGAAGGCTTATGAAAT	PmlI – EcoNI
215–242 mut	CAGCATTGATCGATACCTGGCTATTGTTCACCCAATGAAGTCCCGCCTTC GACGCACAATGCTTGTAGCCAAAGTCACCTGCATCATCATTTGGCTGCTG GCAGGCTTGGCCAGTTTGCCAGCTATAATCCATCGAAATGTATTTTTCATT GAGAACACCAATATTACAGTTTGTGCTTTCCATTATGAGTCCCAAAATTC AACCCTCCCGATAGGGCTGGGCCTGACCAAAAATATACTGGGTTTCCTGT TTCCTTTTCTGATCATTCTTACAAGTTATACTCTTATTGCGAAGGCCCTAA AGAAGGCTGCTGAAATTCAGAAGAACCAACCAAGAAATGATGATATTGC TAAGATAATTATGGCAATTGTGCTTTTCTTTTTCTTTTCCTGGATTCCCCAC CAAATATTCACTTTTCTGGATGTATTGATTCAACTAGGCATCATACGTGAC TGTAGAATTGCAGATATTGTGGACACGGCCATGCCTATCACCATTTGTAT AGCTTATTTTAACAATTGCCTGAATCCTCTTTTTTATGGCTTTCTGGGGAA AAAATTTAAAAGATATTTTCTCCAGCTTCTAAAATATATTCCCCCAAAAG CCAAATCCCACTCAAACCTTTCAACAAAAATGAGCACGCTTTCCTACCGC CCCTCAGATAATGTAAGCTCATCCACCAAGAAGCCTGCACCATGTTTTGA GGTTGAGTGACTCGAGCATGCATC	ClaI – XhoI
309–327 mut	GAAGGCCCTAAAGAAGGCTTATGAAATTCAGAAGAACAAACCAAGAAAT GATGATATTTTTAAGATAATTATGGCAATTGTGCTTTTCTTTTTCTTTTCCT GGATTCCCCACCAAATATTCACTTTTCTGGATGTATTGATTCAACTAGGCA TCATACGTGACTGTAGAATTGCAGATATTGTGGACACGGCCATGCCTATC ACCATTTGTATAGCTTATTTTAACAATTGCCTGAATCCTCTTTTTTATGGC TTTCTGGGGAAAAAAGCTAAAAGATATGCTCTCCAGCTTCTAAAAGCTAT TCCCCCAAAAGCCCAATCCCACTCAAACCTTTCAACAAAAATGAGCACGC TTTCCTACCGCCCCTCAGATAATGTAAGCTCATCCACCAAGAAGCCTGCA CCATGTTTTGAGGTTGAGTGACTCGAGCATGCATC	EcoNI – XhoI