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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Virol Methods. 2018 Apr 6;257:22–28. doi: 10.1016/j.jviromet.2018.04.005

Figure 4.

Figure 4

Detection of virus in culture media in the Aptamer-based RT Detection Assay. (A) HIV-1 virus was prepared from plasmid pNL4-3 using 293T cells as described in Materials and Methods. Cell-free media was assayed for infectious virus using a limit-dilution assay on HeLa TZM-bl cells in order to calculate TCID50 values. One μl of cell media from non-transfected 293T cells was used for the “0” virus control. Note that for HIV-1, there are typically several thousand virus particles for each TCID50 unit and values are highly dependent on the assay procedure used for quantitation. (B) An assay with purified HIV RT is shown for comparison. See Materials and Methods and Fig. 1 for experimental details.