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. 2018 May 14;12:130. doi: 10.3389/fncel.2018.00130

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Copyright © 2018 Falleroni, Torre and Cojoc.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of Piezo1 channels in NG108-15 cells and the effect of Gsmtx-4 on mechanically activated Ca²⁺ transient. (A) NG108-15 cells at 2DIV stained for Piezo 1 (green) and Hoechst 33342 nuclear stain (blue). (B) Brightfield and DF/F image obtained before and after the mechanical stimulation. (C) Time course of the evoked calcium transient (green trace) from white ROI in (B). The blue lines indicate the application of the mechanical force. (D) Bar graphs represent fluorescence changes of Ca²⁺ (DF/F) in untreated cells stimulated with one pulse of strength k, as control group (n = 8) vs. cells treated with Gsmtx-4 (n = 10).