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. 2018 May 14;9:508. doi: 10.3389/fphys.2018.00508

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Copyright © 2018 Martinez-Pinna, Soriano, Tudurí, Nadal and de Castro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The chloride channel blocker 9AC diminishes firing frequency in both Ph and T neurons. (A) Repetitive firing of a Ph (left) neuron in control solution after the injection of 0.5 nA depolarizing current (black upper traces) and its modification after the addition of 2 mM 9AC (blue lower traces). The same effect in a T neuron (right). The injected current protocol is shown in each recording below the membrane potential. The RMP of cells is indicated at the beginning of each recording. Calibration bars apply to both panels. (B) Significant reduction of firing frequency elicited with 1.0 nA depolarizations in the presence of 2 mM 9AC for both populations of Ph (n = 9) and T sympathetic neurons (n = 9). Paired t-test: ^∗P < 0.05; ^∗∗P < 0.01.