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. 2018 May 14;9:464. doi: 10.3389/fphys.2018.00464

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Copyright © 2018 Cuenca, Le-Gatt, Castillo, Belletti, Díaz, Kurte G, Gonzalez, Alcayaga-Miranda, Schuh, Ezquer, Ezquer and Khoury.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MenSCs exhibited a robust wound healing transcriptomic pattern. MSCs were cultured in basal conditions (medium serum free) and with pro-inflammatory cytokines, IL1β and TNFα (10 ng/ml each) during 24 h. The cells were recovered and total mRNA was extracted, reverse-transcribed and RT-qPCR was performed using Stratagene Mx3000P. The quantitative data from the RT-PCR was normalized by the expression of GAPDH. Expression of different genes involved in wound healing process, (A) fold induction of MenSCs vs. UC-MSCs and IL1β+TNFα MenSCs vs. IL1β+TNFα UC-MSCs and (B) fold induction of IL1β+TNFα MenSCs vs. MenSCs and IL1β+TNFα UC-MSCs vs. UC-MSCs. Values are expressed as the mean ± SE of at least 3 independent experiments in triplicates. ^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001 compared to their corresponding MSCs condition. (C) GO analysis showing the 10 most important biological process of IL1β+TNFα MenSCs vs. MenSCs and IL1β+TNFα UC-MSCs vs. UC-MSCs. MSCs, mesenchymal stem cells; MenSCs, menstrual derived mesenchymal stem cells; UC-MSCs, umbilical cord-derived mesenchymal stem cells; TNFα, tumor necrosis factor alpha; IL1β, interleukin 1 beta.