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. 2018 May 14;9:633. doi: 10.3389/fpls.2018.00633

FIGURE 2.

FIGURE 2

Identification of cas31 mutants. (A) Identification of the retrotransposon Tnt1-insertion mutant, NF5714. Tnt1-insertion was localized in the third exon of MtCAS31 (i) and the full length of MtCAS31 cannot be detected by RT-PCR (ii) ACTIN was reference. (B) Identification of the cas31 mutants generated by transcription activator-like effector nuclease (TALEN) technology. Deletions were 40, 25, 8 bp in the cas31-27, cas31-34, and cas31-51 knockout mutants, respectively. (C) Relative expression of MtCAS31 in cas31 mutant by qRT-PCR. Values were normalized to ACTIN expression. (D) Immunoblotting analysis of WT and cas31 mutants with anti-MtCAS31 antibody. ACTIN was reference. (E) Phenotype of WT and cas31 mutants under drought stress. (F) Relative water content (RWC) in WT and cas31 mutant under drought stress. Data represent the mean ± SD (n = 30). Significance is indicated by letters; one-way ANOVA, LSD.