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. 2018 May 14;9:931. doi: 10.3389/fmicb.2018.00931

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Copyright © 2018 Hücker, Vanderhaeghen, Abellan-Schneyder, Scherer and Neuhaus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Promoter activity of the region upstream of the TSS under anaerobic conditions. At an OD₆₀₀ of 0.5, the fluorescence caused due to the pProbe-NT-PromoterTSS plasmid was measured. As negative control, the fluorescence of Escherichia coli transformed with pProbe-NT was also determined, which was 289 ± 110 (not shown in the diagram). The fluorescence value of pProbe-NT-PromoterTSS was at all conditions significantly higher than the vector control (p < 0.001). Significant changes between 0.5×LB and investigated stress conditions were calculated by Student’s t-test and marked with asterisks (^∗∗p < 0.01, ^∗∗∗p < 0.001).