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. 2018 May 14;9:931. doi: 10.3389/fmicb.2018.00931

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Copyright © 2018 Hücker, Vanderhaeghen, Abellan-Schneyder, Scherer and Neuhaus.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic tree and sequence alignment of Ano. The phylogenetic tree on the left was constructed for representative species possessing homologs of ano or ECs2385, antisense overlapping to each other. The tree is based on a concatemer of 16S rRNA, atpD, adk, gyrB, purA, and recA. On the right, the different amino acid sequences of Ano (if present) are aligned (black). Translated start codons are colored in green and untranslated stop codons in red (^∗). Variable regions with no detectable amino acid homology to Ano are colored in blue.